Data Sheet 1_Salidroside inhibits glioma cell migration and proliferation through CYP2E1-neuroglobin-cytochrome c mediated redox axis.docx

The mechanism of Ngb aided apoptosis was investigated by protein expression of Ngb, caspase 3 and cytosolic cytochrome c in glioma cells.</p>Results<p>Ngb expression in glioma tissues samples significantly downregulated with increasing tumour grade as determined by immunoreactivity and western blot analysis. In-silico analyses predicted a possible interaction of salidroside with reduced state of Ngb, In vitro studies suggested significant reduction in cell viability, migration and invasion in both U87MG and LN229 cells on supplementation with salidroside.

These effects were accompanied by increased CYP2E1 activity, altered intracellular NADPH levels indicating induction of oxidative stress. Mitochondrial dysfunction, manifested as reduced MMP, elevated lipid peroxidation and increased cytosolic cytochrome c release indicated activation of intrinsic apoptotic pathway. Computational analyses additionally predicted a preferential interaction with the reduced form of Ngb, providing a mechanistic hypothesis warranting experimental validation.</p>Conclusion<p>Salidroside exerts multi-faceted anti-cancer effects in glioblastoma cells, potentially involving upregulation of Ngb levels in glioma cells, modulation of redox homeostasis, mitochondrial integrity and intrinsic apoptotic signaling.</p>