Data · collection · 2018
CAREER: Protist Nutritional Strategies in Permanently Stratified Antarctic Lakes
Listed in NASA Earthdata CMR
This project supported an integrated research and education program in the fields of polar biology and environmental microbiology, focusing on single-celled eukaryotes (protists) in high latitude ice-covered Antarctic lakes systems.
Description
Protists play important roles in energy flow and material cycling, and act as both primary producers (fixing inorganic carbon by photosynthesis) and consumers (preying on bacteria by phagotrophic digestion).
The McMurdo Dry Valleys (MDV) located in Victoria Land, Antarctica, harbor microbial communities which are isolated in the unique aquatic ecosystem of perennially ice-capped lakes. The project studied: (1) the impact of permanent biogeochemical gradients on protist trophic strategy, (2) the effect of major abiotic drivers (light and nutrients) on the distribution of two key mixotrophic and photoautotrophic protist species, and (3) the effect of episodic nutrient pulses on mixotroph communities in high latitude (ultraoligotrophic) MDV lakes versus low latitude (eutrophic) watersheds.
Read the rest (3 more)
Sampling dates: February 4 – April 10, 2008; November 11- 28, 2012; December 12, 2012 Sampling locations/depths: East Lobe Lake Bonney/5m, 10m, 13m, 15m, 20m, 25m, 30m West Lobe Lake Bonney/5m, 10m, 13m, 15m, 20m, 25m, 30m Lake Fryxell/5m, 7m, 9m, 11m, 12m, 15m Lake Vanda/10m, 20m, 30m, 40m, 50m, 60m, 70m, 75m, 80m Two kinds of metadata from this project are available: 1) DNA sequence data – DNA was extracted from filtered lake water (1-2L) collected from sampling locations and dates reported above.
Environmental DNA was PCR-amplified using primers specific for the following genes: 16S rRNA, 18S rRNA, rbcL, cbbM, nifJ, psbA. Genes were sequenced on an Applied Biosystems DNA analyzer or an Illumina MiSeq or HiSeq instruments. All DNA sequences from this project are available via GenBank. 2) Limnological metadata - Limnological data was collected from sampling locations and dates reported above.
Data includes PAR, conductivity, temperature, Chlorophyll a, and macronutrients and is available via the McMurdo Dry Valleys LTER Data Center.
Links
Get the data
- doi.org /10.1111/1574-6941.12296 ↗
(3) JQ9243533-JQ924384 - description: psbA DNA sequences from Sanger; DOI: https://doi.org/10.1111/1574-6941.12296
download · from NASA CMR
- doi.org /10.1002/lno.10071 ↗
(4) KJ848331-KJ848439 - description: rbcL, cbbM, nifJ DNA sequences from Sanger; DOI: https://doi.org/10.1002/lno.10071 KU196097-KU196166 - description: 18S rRNA sequences from Illumina; DOI: 10.1128/
download · from NASA CMR
- doi.org /10.1007/s00300-016-2025-8 ↗
(5) PRJNA396917 - description: 16S rRNA sequences from Illumina; DOI: https://doi.org/10.1007/s00300-016-2025-8.
download · from NASA CMR
- doi.org /10.1038/nature24621 ↗
(6) ERS1471338-1471434 - description: 16S rRNA sequences from Illumina; DOI: DOI: 10.1038/nature24621
download · from NASA CMR
- mcmlter.org ↗
Environmental Data: Accompanying limnological data (PAR, conductivity, temperature, Chlorophyll a, nutrients) is available through the McMurdo Dry Valleys LTER Data Center.
download · from NASA CMR
- doi.org /10.1111/j.1574-6941.2012.01431.x ↗
JN091926-JN091960 - description: cbbM DNA sequences from Sanger; DOI: https://doi.org/10.1111/j.1574-6941.2012.01431.x
download · from NASA CMR
- ncbi.nlm.nih.gov /genbank ↗
Genetic sequence data is available through NCBI. Sequence Accession Numbers are: GU132860-GU132939 - description: rbcL DNA sequences from Sanger; DOI: 10.1128/AEM.00029-12
download · from NASA CMR
Documentation and papers
- NSF Award Abstract nsf.gov/awardsearch/showAward.do?AwardNumber=1056396 ↗
documentation · from NASA CMR
Catalogue records · 2
- CMR record cmr.earthdata.nasa.gov/search/concepts/C2532071892-AMD_USAPDC.html ↗
catalogue entry · from NASA CMR
- CMR UMM-JSON cmr.earthdata.nasa.gov/search/concepts/C2532071892-AMD_USAPDC.umm_json ↗
metadata API · from NASA CMR
Topics
- Stated by source
- Community Dynamics · Freshwater Ecosystems · NOT APPLICABLE · NOT APPLICABLE
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Earthdata CMR | C2532071892-AMD_USAPDC | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[instrument].gcmd_instrument:not-applicable | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/biosphere/ecological-dynamics/community-dynamics | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/biosphere/ecosystems/freshwater-ecosystems | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[platform].gcmd_platform:not-applicable | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| created_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| description | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/Abstract |
| publication_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| spatial | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/SpatialExtent |
| temporal | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/TemporalExtents |
| title | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/EntryTitle |
| updated_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| version_label | source · NASA CMR | connector:nasa_cmr@1.0.0 |