52 datasets

NCBI GEO2026 · RNA sequencing · 2 samples

…To accurately illustrate the Changes in the Liver during heart allograft rejection, we established the murine heterotopic heart transplantation model and harvested liver of recipients… …for single cell transcriptome sequencing.…

NCBI GEO2026 · RNA sequencing · 9 samples

…We profiled over 34,000 cells from resected zebrafish hearts spanning 10 developmental stages from 25 hours to 60 days post-fertilization.… …This zebrafish heart atlas enhances our understanding of vertebrate heart development and represents a valuable resource for future studies.…

NCBI GEO2026 · RNA sequencing · 90 samples

…Results: We generate an integrated multiomic atlas of human cardiac cells, combining newly generated and publicly available single-nucleus RNA sequencing datasets from 299 donors and… …single nucleus ATAC-seq datasets from 106 donors.…

NCBI GEO2026 · RNA sequencing · 8 samples

…This transformation requires precise spatial and temporal coordination among diverse cardiac cell types; central to this process is cardiomyocyte maturation, yet the regulatory mechanisms… …Here, we present a temporal and spatial atlas of postnatal hearts by integrating single-nucleus transcriptomics with image-based spatial transcriptomics, which uncovers the dynamic…

NCBI GEO2026 · RNA sequencing · 3 samples

…This transformation requires precise spatial and temporal coordination among diverse cardiac cell types; central to this process is cardiomyocyte maturation, yet the regulatory mechanisms… …Here, we present a temporal and spatial atlas of postnatal hearts by integrating single-nucleus transcriptomics with image-based spatial transcriptomics, which uncovers the dynamic…

NCBI GEO2026 · RNA sequencing · 2 samples

…To determine the activation of PD1+ T cells in MI, we sorted and sequenced PD1+ T cells accompanied with PD1- T cells in the mice heart after MI.… …In order to deeply investigate the function of PD1+ T cells in heart during MI, we performed single-cell RNA sequencing (scRNA-seq) to detect the change T cells from anti-IgG and anti-PD1…

NCBI GEO2026 · RNA sequencing · 3 samples

…Understanding which factors contribute for zebrafish heart regeneration is crucial for developing regeneration-inducing strategies as mammalian hearts are limited in regenerative capacity… …Exploring further our recently published optimized approach for increasing the single cell RNA sequencing (scRNA-seq) resolution specifically CMs, we have systematically evaluated transcription…

NCBI GEO2026 · RNA sequencing · 4 samples

…Here, we established in vivo single-cell RNA metabolic labeling in the adult heart to systematically identify the cells that first respond to injury, as well as the processes that are… …In conjunction with detailed dissection of single-cell transcriptomics atlases, this finding led us to postulate that macrophage-specific inhibition of Toll-like receptor signaling…

NCBI GEO2026 · RNA sequencing · 15 samples

…Here, we established in vivo single-cell RNA metabolic labeling in the adult heart to systematically identify the cells that first respond to injury, as well as the processes that are… …In conjunction with detailed dissection of single-cell transcriptomics atlases, this finding led us to postulate that macrophage-specific inhibition of Toll-like receptor signaling…

NCBI GEO2026 · RNA sequencing · 2 samples

…While pluripotent stem cell-derived heart organoids model cardiomyocyte differentiation, they do not recapitulate early morphogenetic events.… …Single-cell transcriptomics and trajectory analyses, together with pulse-labeling, support a model in which progressive incorporation of cardiac cells from proliferative splanchnic…

NCBI GEO2026 · RNA sequencing · 6 samples

…Single cell or single nuclei RNA-sequencing (snRNAseq) enables high-resolution profiling of cellular states, but sample preparation is often costly and complicated by debris formation… …and cell clumping.…

NCBI GEO2026 · RNA sequencing · 12 samples

…The Mexican cavefish provides a unique comparative model to study heart regeneration and scarring within a single species, with Surface fish capable of heart regeneration and their… …Using single-cell transcriptomics and immune perturbations, we find significant differences in the immune response between the two populations.…

NCBI GEO2026 · RNA sequencing · 4 samples

…Programmed cell death protein 1 (PD1) is crucial for adaptive immunity regulation, showing considerable effects in various diseases especially in tumor field.… …In order to deeply investigate the expression characteristics of PD1+ T cells in heart during myocardial infarction (MI), we firstly performed single-cell RNA sequencing (scRNA-seq)…

NCBI GEO2026 · RNA sequencing · 2 samples

…It is well established that lymphatic vessels regulate interstitial fluid homeostasis and facilitate the transport of macromolecules and immune cells.… …Single-cell RNA sequencing revealed that, during heart regeneration, ackr3a mutants exhibit altered LEC subcluster compositions and differential expression of several candidate downstream…

NCBI GEO2026 · RNA sequencing · 4 samples

…Pressure overload_induced heart failure is associated with extensive cellular remodeling in the heart.… …To investigate the cell type_specific transcriptional responses regulated by miR_210, single_nucleus RNA sequencing (snRNA_seq) was performed on heart tissues collected from wild_type…

NCBI GEO2026 · RNA sequencing · 6 samples

…Single-nucleus RNA sequencing (snRNA-seq) enables transcriptomic profiling of complex tissues such as the heart, where dissociation into intact single cells is challenging.… …All platforms consistently resolved major cardiac cell populations.…

NCBI GEO2026 · RNA sequencing · 18 samples

…Early postnatal heart growth requires a transition from cardiomyocyte proliferation to enlargement and binucleation.… …Using morphometric, transcriptomic, signaling and single-cell analyses in neonatal mice, we show that this transition occurs predominantly between postnatal days 3.5 and 4.5 (P3.5-P4.5…

NCBI GEO2026 · Image data · 10 samples

…In this study, we leverage single-cell RNA sequencing and spatial transcriptomics of human coronary arteries to transcriptionally characterize CAV and define the neointimal microenvironment… …Integration of single-cell RNA sequencing and spatial transcriptomic datasets revealed that modulated vascular smooth muscle cells and macrophage subsets dominate the CAV neointima…

NCBI GEO2026 · RNA sequencing · 2 samples

…We assayed single-cell gene expression and chromatin accessibility to generate comprehensive genome-wide regulatory maps.… …Single-cell RNA-seq of EHTs confirmed populations recapitulating major cell types found in hearts, with advanced cardiomyocyte maturation compared to monolayer hiPSC-cardiomyocytes.…

NCBI GEO2026 · RNA sequencing · 2 samples

…To elucidate the role of CD40 in the post-MI repair process, we dissected infarct and border zones from the hearts of wild-type (WT) and CD40 knockout (KO) mice at 3 days post-MI.… …Mononuclear macrophages (CD45⁺CD11b⁺CD64⁺) were isolated via flow cytometry and subjected to single-cell RNA sequencing analysis.…

NCBI GEO2026 · RNA sequencing · 4 samples

…Single-cardiac-endothelial-cell transcriptomics and lineage-tracing system reveal that inflammatory CMECs subcluster activation is responsible for the deteriorating HFpEF progression… …This study uncovers that activation of SR-B1–CXCL10-CXCR3 axis in CMECs aggravates HFpEF pathogenesis through accumulation of CXCR3-positive T-cells in hearts.…

NCBI GEO2026 · RNA sequencing · 1 samples

…We employed single-cell RNA sequencing (scRNA-seq) in a mouse model of MI to gain a detailed analysis of heart and lymphoid Tregs, focusing on their developmental trajectories.… …the mediastinal lymph node (mLN) and heart after MI identified three Treg populations, one of which was mainly derived from the heart.…

NCBI GEO2026 · RNA sequencing · 6 samples

…Heart is one of the most vulnerable target organs in sepsis.… …Therefore, using single-cell RNA sequencing to clarify the mechanisms of SIMD is of great significance to effectively prevent sepsis shock from developing into multiple organ dysfunctions…

NCBI GEO2026 · RNA sequencing · 15 samples

…We assayed single-cell gene expression and chromatin accessibility to generate comprehensive genome-wide regulatory maps.… …Single-cell RNA-seq of EHTs confirmed populations recapitulating major cell types found in hearts, with advanced cardiomyocyte maturation compared to monolayer hiPSC-cardiomyocytes.…

NCBI GEO2026 · RNA sequencing · 2 samples

…We used a 10X Genomics single-cell RNA sequencing approach to compare the single-cell transcriptome of heart ventricles from sulf1sjr9-/- at uninjured state and at 4 days post-cryoinjury…