Omics · study · 2026
Effects of Rg3@PACVs with NIR in a Rat Model of Pulmonary Ischemia–Reperfusion Injury
Listed in NCBI GEO
Pulmonary ischemia–reperfusion injury (IRI) is characterized by excessive inflammation, oxidative stress, and mitochondrial dysfunction, leading to severe lung damage.
Description
To elucidate the molecular mechanisms underlying the protective effects of a macrophage membrane-coated mesoporous polydopamine nanoparticle system loaded with Ginsenoside Rg3 (Rg3@PACVs) combined with near-infrared (NIR) irradiation, we performed transcriptome sequencing of rat lung tissues.
Differential gene expression profiles were compared between IRI and IRI + Rg3@PACVs + NIR groups. A total of 209 upregulated and 332 downregulated genes were identified in the treatment group relative to IRI controls. Functional enrichment analyses revealed that Rg3@PACVs + NIR pretreatment enhanced pathways related to endoplasmic reticulum stress response, PI3K-AKT signaling, oxygen transport, protein folding, hypoxia response, TCA cycle activity, ATP-dependent processes, and cell cycle progression.
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In contrast, inflammation-associated pathways including TLR/NF-κB signaling, cytokine binding, cell adhesion molecules, neutrophil extracellular trap formation, IL-1β production, and leukocyte trans-endothelial migration were significantly suppressed. GSEA further demonstrated reduced oxidative stress (cytochrome P450 activity) and enhanced FOXO signaling. These findings indicate that Rg3@PACVs + NIR mitigates pulmonary IRI by coordinately suppressing inflammatory responses, alleviating oxidative stress, and restoring mitochondrial homeostasis, thereby promoting lung tissue repair.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324247 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324247 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1434304 ncbi.nlm.nih.gov/bioproject/PRJNA1434304 ↗
project · from NCBI GEO
- PubMed 42030937 pubmed.ncbi.nlm.nih.gov/42030937 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE324247 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |