Total RNA seq in the human control and faling hearts

Differential expression analyses were conducted using the cufflinks and the DESeq2 algorithm. Results: An average of 211 million reads of total-RNA-seq librairies were generated. From these data, we identified a panel of 3247 lncRNAs that were either cardiac-enriched or differentially expressed between failing and non-failing hearts identified by either Cufflinks or DESeq2, including 1036 novel lncRNAs (i.e. not described in the GENCODE version 19 database). 232 of them were derived from novel genes and were from moderately to highly expressed in at least half samples.

The novel gene derived lncRNAs differentially expressed in failing heart could correlate to at least one heart failure associated genes with a Spearman's rank correlation coefficient more than 0.7 or less than -0.7 in either ICM or DCM group.