Bouwen et al Nat Comm 2025 scRNAseq

I7_1382_01 Non seizure - I17-1382-01-H072_cells_S24_L001_R1_001.fastq.gz - I17-1382-01-H072_cells_S24_L002_R1_001.fastq.gz I7_1425_01 Non seizure - I17-1425-01-H078_PCs_S6_L001_R1_001.fastq.gz - I17-1425-01-H078_PCs_S6_L002_R1_001.fastq.gz I8_1031_01 Non seizure - I18-1031-01-H085_PCs_S49_L001_R1_001.fastq.gz - I18-1031-01-H085_PCs_S49_L002_R1_001.fastq.gz I8_1105_01 Seizure - I18-1105-01-H089_PCs_S40_L001_R1_001.fastq.gz - I18-1105-01-H089_PCs_S40_L002_R1_001.fastq.gz I8_1191-01 Non seizure - I18-1190-01-H088_PCs_S38_L001_R1_001.fastq.gz - I18-1190-01-H088_PCs_S38_L002_R1_001.fastq.gz I8_1192_01 Seizure - I18-1192-01-H093_PC_S18_L001_R1_001.fastq.gz - I18-1192-01-H093_PC_S18_L002_R1_001.fastq.gz I8_1201_01 Seizure - I18-1201-01-H094_PC_S4_L001_R1_001.fastq.gz - I18-1201-01-H094_PC_S4_L002_R1_001.fastq.gz I8_1425_01 Non seizure - I18-1425-01_S25_L001_R1_001.fastq.gz - I18-1425-01_S25_L002_R1_001.fastq.gz I8_1430_01Non seizure - I18-1430-01_S26_L001_R1_001.fastq.gz - I18-1430-01_S26_L002_R1_001.fastq.gz I8_1455_01 Seizure - I18-1455-01_S3_L001_R1_001.fastq.gz - I18-1455-01_S3_L002_R1_001.fastq.gz I9_1026_01Non seizure - I19-1026-01_S11_L001_R1_001.fastq.gz - I19-1026-01_S11_L002_R1_001.fastq.gz I9_1035_01 Seizure - I19-1035-01_S34_L001_R1_001.fastq.gz - I19-1035-01_S34_L002_R1_001.fastq.gz Patch-seq using Smart-seq2 protocol for human neurons In a sub set of patient samples we harvested pyramidal cells for modified patchSeq analysis using the protocol for Smart-seq2.

From sliced cortical samples, cell contents were harvested and processed according to the methods as described. Next, single-cell mRNA was converted to cDNA and sequencing libraries were created. DNA libraries for sequencing were prepared from cDNA libraries with the Illumina Nextera XT kit.