Single-Cell RNA Sequencing Identifies CXCL9:SPP1 Polarization as a Driver of NAFLD Progression—Supplementary Materials

The supplementary methods describe scRNA-seq data quality control, integration, dimensionality reduction, cell type and subcluster annotation, and antibody information for immunofluorescence experiments.

The supplementary results present global quality control and batch-effect assessment of scRNA-seq data, marker expression features for major cell type and subcluster annotation, GO–KEGG enrichment analysis of CD80+ KCs and Spp1+ KCs, and external validation of CS polarization using an independent single-cell dataset.