Omics · study · 2026
RNA-seq of human iPS cell-derived retinal ganglion cells
Listed in NCBI GEO
The integrin family is a key regulator of cellular behavior, transducing adhesion signals through interactions with the extracellular matrix (ECM).
Description
Despite its central role in retinal ganglion cell (RGC) survival and neurite outgrowth, the comprehensive expression profile of integrins in human RGCs remains poorly characterized. In this study, we performed RNA sequencing on human iPSC-derived RGCs to elucidate their global transcriptomic landscape.
Following a modified Sluch et al. (2017) protocol, RGCs were differentiated from 201B7 iPSCs and isolated at day 40 via CD90.2/Thy1.2-targeted magnetic-activated cell sorting (MACS). Our analysis confirmed robust expression of canonical RGC markers and revealed a significant enrichment of genes involved in ECM-mediated signaling, providing new insights into the molecular basis of RGC-scaffold interactions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326418 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326418 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1445227 ncbi.nlm.nih.gov/bioproject/PRJNA1445227 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 71% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE326418 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |