Omics · study · 2026
Small RNA sequencing of whole blood from a rat model of chronic primary pain with adrenergic receptor blockade
Listed in NCBI GEO
The sympathetic nervous system has been implicated in the development of chronic primary pain conditions (CPPCs), yet the downstream molecular mechanisms linking sustained catecholamine signaling to chronic pain remain poorly understood.
Description
Here, we profiled circulating small RNAs in a rat model of CPPC induced by sustained catecholamine elevation through catechol-O-methyltransferase (COMT) inhibition. Female Sprague-Dawley rats (N = 5/group) were assigned to four treatment groups: Vehicle + Vehicle (pain-free control), OR486 + Vehicle (primary pain condition), Vehicle + ICI-118,551/SR59230A, or OR486 + ICI-118,551/SR59230A.
OR486 and vehicle were delivered continuously for 14 days by subcutaneous osmotic minipump. Separate minipumps delivered the ADRB2-selective antagonist ICI-118,551 and the ADRB3-selective antagonist SR59230A or vehicle. Whole blood was collected on Day 14 for small RNA isolation and Illumina small RNA sequencing.
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Differential expression analysis identified a discrete set of circulating miRNAs altered during primary pain, including significant downregulation of miR-133a-3p and upregulation of multiple inflammation-associated miRNAs. Pharmacologic blockade of ADRB2/3 prevented miR-133a-3p downregulation, identifying miR-133a-3p as an adrenergic receptor-dependent regulator associated with chronic primary pain.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338452 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338452 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1494513 ncbi.nlm.nih.gov/bioproject/PRJNA1494513 ↗
project · from NCBI GEO
- PubMed 42479461 pubmed.ncbi.nlm.nih.gov/42479461 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Non-coding RNA profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE338452 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:non-coding-rna-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |