Small RNA Sequencing of Exosomal MicroRNAs in Sera from Patients with Echinococcus granulosus Infection

First, serum exosomes were isolated and purified via size-exclusion chromatography with strict quality control, followed by morphological identification of exosomes using negative-staining transmission electron microscopy.

Subsequently, exosomal RNA was extracted, and small RNA libraries were constructed with the Illumina TruSeq Small RNA Kit for single-end 50 bp sequencing on the Illumina Hiseq 2000/2500 platform. Raw sequencing data were processed and analyzed through the ACGT101-miR pipeline, including clean data generation, sRNA length screening, database alignment for filtration, miRNA identification, and further analysis of differentially expressed miRNAs as well as their target gene prediction and functional enrichment.