Omics · study · 2026
The NAMPT/SIRT7/SOD2 axis regulates alveolar progenitor cell renewal and pulmonary fibrosis [scRNA-seq]
Listed in NCBI GEO
We employed single-cell RNA sequencing using the 10x Genomics platform to investigate the role of nicotinamide phosphoribosyltransferase (NAMPT), a key enzyme in NAD+ biosynthesis, in AT2 cells in the lung.
Description
NAMPT has been implicated in regulating stem cell function, mitochondrial bioenergetics, apoptosis, and senescence in neurodegenerative diseases, endothelial cells, and human iPS cells. Single-cell lung suspensions were prepared from Nampt AT2 conditional knockout and control mice at day 0 and day 4 post-bleomycin injury. scRNA-seq was performed on FACS-sorted Epcam+ and CD45+ live cells, and purified cells were clustered to evaluate NAMPT function in AT2 and immune cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295739 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295739 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1256279 ncbi.nlm.nih.gov/bioproject/PRJNA1256279 ↗
project · from NCBI GEO
- PubMed 42096291 pubmed.ncbi.nlm.nih.gov/42096291 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE295739 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |