RR-1 and RR-3 mouse liver transcriptomics with and without ERCC control RNA spike-ins

To this end, the External RNA Control Consortium (ERCC) developed a standard set of 92 poly-adenylated RNA transcripts that are orthogonal to mammalian RNA that can be added to RNA extracts before library generation and sequencing. The presence of these RNA standards at known ratios improves interpretation of RNA sequencing datasets.

To test the utility of the ERCC RNA controls, total RNA extracted from mouse livers from the Rodent Research 1 (flight, ground control, basal control and vivarium control groups) and Rodent Research 3 (flight, ground control and basal control groups) missions were sequenced with and without the ERCC control RNA (RR-3 liver samples without ERCC control have been sequenced previously and the dataset is released in the GLDS-137).