Data · dataset · 2021
Ectomycorrhizal and arbuscular mycorrhizal fungi associated with aspen (Populus tremuloides) roots yielded from Illumina sequencing
Listed in Borealis and Agri-environmental Research Data Dataverse — shown once because both records carry DOI 10.7939/dvn/vsirog
On August 24, 2019, Justine Karst and Nadir Erbilgin sampled a 30–50 cm segment of root from each of three aspen trees within each plot at the interface of the humus layer of the forest floor and mineral soil.
Description
We traced roots from boles and confirmed their identity to be aspen by taste-testing. Sampled roots were put on ice after collection and transported to the University of Alberta the same day and stored at -20 °C prior to processing.
Aspen roots were thawed and washed free of adhering soil. Fine roots (< 1 mm in diameter) were retained, cut into 1 cm fragments, mixed thoroughly in a dish of water, and subsampled for DNA sequencing of AM and EM fungal communities using Illumina. The ITS1 and SSU regions of fungal rDNA were separately amplified in two-step PCRs using primers ITS1F - ITS2 and WANDA - AML2, respectively.
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James Franklin performed benchwork and Joseph Birch performed bioinformatics.
Links
Where it is published
- Dataverse dataset page borealisdata.ca/dataset.xhtml?persistentId=doi%3A10.7939%2FDVN%2FVSIROG ↗
landing page · from borealisdata ca
- DOI doi.org/10.7939/dvn/vsirog ↗
DOI / persistent id · from borealisdata ca
Catalogue records · 1
- Dataverse API borealisdata.ca/api/datasets/:persistentId/?persistentId=doi%3A10.7939%2FDVN%2… ↗
metadata API · from borealisdata ca
Topics
- From keywords
- Chemistry · Computer Science & AI · Earth & Environmental Science · Economics & Finance · Engineering · Humanities · Life Sciences · Mathematics & Statistics · Medicine & Health · Ocean & Atmospheric Science · Social Science
- Inferred from text
- Evolutionary biology 73% · Sequencing 75%
Provenance · 2 source records, 21 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Borealis | doi:10.7939/DVN/VSIROG | 13 d ago | JSON v1 |
| Agri-environmental Research Data Dataverse | doi:10.7939/DVN/VSIROG | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3104 | enrichment · borealisdata ca | taxonomy-embedding@1.0.0 | title+keywords+description (73%) |
| concepts[field].dataverse_subject:other | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:other | source · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:chemistry | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:computer-science-ai | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:earth-environmental | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:economics-finance | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:engineering | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:humanities | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:mathematics-statistics | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:ocean-atmospheric | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:social-science | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[modality].local:modality:sequencing | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| description | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /description |
| publication_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| title | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /name |
| updated_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| version_label | source · borealisdata ca | connector:borealisdata_ca@1.0.0 |