← Search

Excel · study · 2026

Bulk RNA-sequencing of killifish retina-RPE across aging

Listed in NCBI GEO

Bulk RNA sequencing was performed on retina-retinal pigment epithelium (retina-RPE) tissue collected from male Nothobranchius furzeri (GRZ background) at four ages (1.5, 3.0, 4.5, and 6.0 months).

Description

Total RNA was isolated using the RNeasy Plus Micro Kit (QIAGEN), and sequencing libraries were prepared by Azenta using the Illumina Nextera XT Library Preparation Kit followed by sequencing on an Illumina NovaSeq S4 platform.

These data were generated to characterize age-associated transcriptomic changes in the retina-RPE and serve as an independent dataset for evaluating the MKMC alignment-free transcriptomic analysis framework.

Links

Where it is published

Documentation and papers

Topics

From keywords
Life Sciences
Inferred from text
RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 8 field assertions
SourceKeyLast seenRaw
NCBI GEOGSE33781015 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[field].local:field:life-sciencesmapping · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencingsource · NCBI GEOconnector:ncbi_geo@1.0.0/gdstype
concepts[modality].local:modality:rna-seqenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
concepts[modality].local:modality:sequencingenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
descriptionsource · NCBI GEOconnector:ncbi_geo@1.0.0/summary
publication_datesource · NCBI GEOconnector:ncbi_geo@1.0.0
titlesource · NCBI GEOconnector:ncbi_geo@1.0.0/title