Omics · study · 2026
Gene expression profile in Shh-expressing cells of hair follicles in mouse during CIA
Listed in NCBI GEO
Chemotherapy-induced alopecia (CIA) is a major unsolved adverse effect in clinical oncology.
Description
In previous studies, we proved the Sonic hedgehog(Shh) signaling pathway is a major target of cyclophosphamide (CYP). However, how CYP suppresses Shh gene remains unknown.
In this study, we isolated Shh-expressing cells in hair follicles of Shh-GFP+/- mice treated with CYP and tested the effect of CYP on these cells. The result showed that Shh-expressing cells decreased significantly post-CYP treatment. RNA sequencing data showed that compared with the control group, 536 and 2,439 genes expressed differentially at 12hrs and 24hrs respectively post-CYP treatment.
Read the rest (2 more)
KEGG enrichment analysis revealed the p53 and the apoptosis signaling pathway were significantly enriched at 12hrs and 24hrs after CYP injection. The immunofluorescence staining of active Caspase 3 confirmed the apoptosis of Shh-expressing cells at 12 hours post-CYP treatment. GSEA analysis, ChIP assay, and AG-490 rescue assay revealed the IFN/JAK/STAT1 signaling pathway participated in regulating Shh during CIA.
The results of this study demonstrated the decrease of Shh during CIA not only because of the loss of Shh-expressing cells but also because of the down expression of Shh in Shh-expressing cells. Moreover, this study also proved that apoptosis is the main reason for the loss of Shh-expressing cells, while the IFN/JAK/STAT1 signaling pathway may play an important role in the downregulation of Shh.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE277nnn/GSE277257 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277257 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1161602 ncbi.nlm.nih.gov/bioproject/PRJNA1161602 ↗
project · from NCBI GEO
- PubMed 41263532 pubmed.ncbi.nlm.nih.gov/41263532 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE277257 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |