Activation of donor-derived eosinophils contributes to primary graft dysfunction during static lung graft storage

Cells were incubated at 37°C to mimic rewarming conditions that typically induce activation. Exposure to Perfadex® Plus resulted in reduced cell viability and suppression of eosinophil-associated transcriptional programs related to recruitment, activation, and survival.

In contrast, supplementation with GSH preserved cell viability and significantly enhanced the expression of antioxidant and redox-related pathways. Enrichment analysis revealed activation of oxidative stress response, detoxification, glutathione metabolism, selenium-associated processes, and mitochondrial respiration. These findings suggest that GSH mitigates metabolic stress and dampens pro-inflammatory transcriptional activity in eosinophils, supporting its potential role in improving lung preservation outcomes.