Omics · study · 2026
Mechanotransduction unifies healthy non-diabetic wound healing over time by promoting a Cd14+/C1qa+ fibroblast subpopulation
Listed in NCBI GEO
Diabetes is a major growing public health concern, but the impact on cellular impairments in chronic wounds remains incompletely understood.
Description
Fibroblasts, key players in all phases of wound healing, are particularly responsive to mechanotransduction. Here, we characterize fibroblast heterogeneity in genetically induced and pathophysiological diabetic murine wounding models across all wound healing phases.
Full-thickness excisional wounds were created on the dorsum of C57BL/6 non-diabetic wild-type (WT) mice (N-DB), WT-mice fed a high-fat diet to create pathophysiologic diabetes (P-DB), and leptin-receptor-deficient mice, a genetically induced diabetic model (G-DB). Tissue was submitted for single-cell RNA sequencing at postoperative days (PODs) 0, 2, 7 and 30. Both G-DB and P-DB significantly impaired wound healing compared to N-DB.
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N-DB expressed distinct myeloid-like Cd14+/C1qa+ angiogenic fibroblasts at PODs 2, and 7. Mechanotransduction pathways FAK and MAPK were consistently upregulated in N-DB fibroblasts and depleted in both diabetic models. G-DB upregulated WNT-activated Dpp4+ fibrotic fibroblasts at POD7 compared to N-DB.
Thus, mechanotransduction pathways are present in physiologically healing fibroblasts and are selectively depleted in both pathophysiologic and genetically induced diabetes. In genetically induced diabetes, overactivation of canonical and non-canonical WNT pathways overwhelms cells and impairs healing processes. Transcriptomic insights into these mechanotransducive perturbations may lead to new therapies for diabetic wound repair.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326622 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326622 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1446331 ncbi.nlm.nih.gov/bioproject/PRJNA1446331 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE326622 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |