Acute high-dose irradiation disrupts cell adhesion and Silk-Ovarioid formation in human primary ovarian cells

In total 50 samples collected from 5 patients were sequenced and 5 groups (i.e.,control_t1, control_t24, irradiated_t1, irradiated_t4, irradiated_t24) were included in the study. RNA extraction was performed using the RNeasy Micro Kit (Qiagen, Germany) following the manufacturer’s instructions.

Libraries were prepared using the Illumina Stranded mRNA Prep Ligation protocol (Illumina, USA) according to the manufacturer’s instruction, with 10 ng of RNA used as input. The dataset contains 100 fastq files from paired-end RNA sequencing, compressed with GNUzip (gzip). The dataset also contains one metadata file in xlsx format and one md5sum file in txt format.