Omics · study · 2026
BoNT/A Modulates Microglial Phagocytic Activity in the Hippocampus Revealed by Single-Cell RNA Sequencing
Listed in NCBI GEO
To investigate the impact of Botulinum Neurotoxin A (BoNT/A) on hippocampal cellular landscapes, we performed single-cell RNA sequencing (scRNA-seq) on tissues from Saline-, MPTP-, and MPTP+BoNT/A-treated mice.
Description
We identified and annotated 10 major cell types through canonical marker gene expression. A focused sub-clustering analysis of microglia revealed 11 distinct subpopulations.
Notably, KEGG pathway enrichment analysis identified three subclusters (1, 3, and 5) as phagocytosis-related microglia, enriched in pathways such as lysosome, phagosome, and FcγR-mediated phagocytosis. Our findings demonstrate that BoNT/A plays a definitive role in modulating microglial phagocytic activity in the hippocampus.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308594 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308594 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1331671 ncbi.nlm.nih.gov/bioproject/PRJNA1331671 ↗
project · from NCBI GEO
- PubMed 42263400 pubmed.ncbi.nlm.nih.gov/42263400 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 70% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE308594 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |