Omics · study · 2026
A Time Course Analysis of the electrophysiological and biochemical properties during differentiation of hair cell- like cells in culture
Listed in NCBI GEO
Bone marrow-derived mesenchymal stromal cells (BMSCs) are multipotent cells that have attracted considerable attention in regenerative medicine.
Description
Current in vitro test focus on biochemical assays of hair cell-like cells (HCLCs) derived from BMSCs associated with changes in electrophysiological properties. HCLCs were produced from BMSCs by culturing BMSCs with B27, EGF, FGF, and IGF-1.
Immunocytochemistry (ICC) and double immunofluorescence staining were used to test hair cell-associated markers on day 17 and 21-26. RNA Sequencing studies and scanning electron microscopy (SEM) were also carried out on induced hair cells. Next, we performed whole-cell patch-clamp recording by utilizing current- and voltage-clamp techniques to assess changes in membrane potential and ionic currents during differentiation.
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Immunostaining assay reveals significant expression of myosin VIIA and SOX2 in cultured hair cells on day 21- 26. We have also found 8 enhanced transcripts in differentiated cell genes (Wnt7a, Mgat5b, Myo7a, Pou4f3, SOX2, Atoh1, Map2k3, Actin) using RNA Sequencing. Electrophysiological results indicate that cells undergoing differentiation had an average resting membrane potential (RMP) of -11.93±0.89mV on day 17 and −58.96 ±1.10 mV on days 21-26.
Differentiated HCLCs displayed a mean resting membrane resistance of 171.66 ±29.12 MΩ, membrane time constant of 10.73 ±0.45 ms and membrane capacitance of 0.0625 ±0.0087 pF, following 21-26 days in culture. Our results also showed cultured HCLCs show morphological development and express transcriptomic profile of this cell type. These findings indicate that alterations in RMP may serve as a valuable indicator for distinguishing HCLCs differentiation potential from BMSCs.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305711 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305711 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1307502 ncbi.nlm.nih.gov/bioproject/PRJNA1307502 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 70% · Microscopy 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE305711 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:microscopy | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |