Omics · study · 2026
Developmental Dynamics of Skeletal Muscle Can Be Recapitulated In Vitro from Pig Embryonic Stem Cells
Listed in NCBI GEO
Understanding skeletal muscle development in pigs is required for fundamental developmental biology and its application in biomedical and agricultural industries.
Description
Here, we employed a directed myogenic differentiation approach to guide pig embryonic stem cells (ESCs) through presomitic mesoderm (PSM) and somite (SM) stages, ultimately generating myogenic progenitors (MPs) and mature muscle cells. The core signaling pathways, including WNT/β-catenin and BMP, along with several growth factors, were involved in sequential myogenic lineage induction in pigs.
During differentiation, single-cell RNA sequencing revealed stage-specific transcriptional dynamics with three distinct ‘Myogenic states’, reflecting the in vivo developmental trajectory: PSM progenitors commitment into posterior PSM, posterior PSM transitioning into anterior PSM, and anterior PSM/SM giving rise to dermomyotome/muscle cells. These findings provide a comprehensive in vitro roadmap of pig skeletal muscle development, advancing developmental biology and biotechnology in livestock.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE293nnn/GSE293311 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293311 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1243987 ncbi.nlm.nih.gov/bioproject/PRJNA1243987 ↗
project · from NCBI GEO
- PubMed 42140199 pubmed.ncbi.nlm.nih.gov/42140199 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Sus scrofa
- From keywords
- Life Sciences
- Inferred from text
- Animal production 69% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE293311 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3003 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9823 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |