Omics · study · 2026
Morphological and Single-Cell Analyses of En Bloc Resected Non-Muscle invasive Bladder Cancer Reveal Structural and functional disruption of Tertiary lymphoid Structures
Listed in NCBI GEO
Tertiary lymphoid structures (TLS) have emerged as critical immune niches within the tumor microenvironment across various cancers.
Description
However, their structural organization and functional roles in non–muscle-invasive bladder cancer (NMIBC) remain poorly characterized. In this study, we provide the first detailed characterization of TLS in NMIBC using en bloc resected specimens, enabling high-resolution spatial mapping and quantitative histological assessment across a large cohort of primary tumors (n=97).
Compared with TLS in inflammation-driven cystitis, tumor-associated TLS showed heterogeneous spatial organization, altered immune composition, and less structured follicular dendritic cell (FDC) networks with reduced high endothelial venule density (p<0.0001). TLS density increased with NMIBC grade and stage (p=0.01) and was associated with greater recurrence risk and shorter progression-free survival in high-TLS tumors (p=0.004).
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In paired primary and recurrent tumors (n=16), TLS showed progressive FDC disorganization and M2 macrophage polarization, supporting their role in shaping an immunosuppressive microenvironment. Single-cell RNA sequencing of TLS from NMIBC and cystitis samples revealed impaired germinal center activity in tumor-associated TLS, with asynchronous B cell maturation and reduced B cell interactions with both T follicular helper and FDC (p<0.0001).
They also showed increased myeloid infiltration and altered dendritic cell function, including reduced MHC class II antigen presentation and downregulation of co-stimulatory (CD86–CD28) and migration-related (CD99) pathways compared to cystitis (p<0.0001). Overall, NMIBC-associated TLS are structurally and functionally impaired, which may limit effective local immune responses and highlights their potential as biomarkers and targets for immunomodulatory therapy in bladder cancer
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310802 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310802 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1367092 ncbi.nlm.nih.gov/bioproject/PRJNA1367092 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE310802 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |