A dataset of methylation profiles in EPC2 cells treated with 3% polycyclic aromatic hydrocarbons (PAHs) and wildtype EPC2 cells

The resulting DNA fragments are then subjected to end repair, A-tail addition, and ligation with sequencing adapters that are modified at all cytosines to ensure they are methylated. Gel electrophoresis is used to select DNA fragments with insert sizes ranging from 150 to 300 base pairs.

The fragments are then treated with bisulfite (using the EZ DNA Methylation Gold Kit, Zymo Research). During this treatment, unmethylated cytosines are converted to uracil (which becomes thymine after PCR amplification), while methylated cytosines remain unchanged. Finally, PCR amplification is performed to generate the final DNA library.Devices: Illumina HiSeq PE150Data processing: Sequencing adaptors and low-quality reads were trimmed from the raw sequencing data to obtain clean data for subsequent analysis.