Table 6_T-cell and monocyte DNA methylation and Wnt dysregulation in common variable immunodeficiency: an exploratory study.xlsx

Background<p>Many patients with common variable immunodeficiency (CVID) develop inflammatory/autoimmune complications associated with T-cell and monocyte dysregulation which may involve epigenetic modifications.</p>Objective<p>To characterize genome-wide CpG methylation in CD3⁺ T-cells and CD14⁺ monocytes in CVID and relate epigenetic alterations to gene expression and circulating proteins.</p>Methods<p>Whole-genome bisulphite sequencing was performed on purified CD3⁺ T-cells and CD14⁺ monocytes from three CVID patients with inflammatory complications and three healthy controls per cell type.

Differentially methylated cytosines were identified and analysed by pathway over-representation. Selected pathway-related proteins were measured in plasma from 60 CVID patients and 30 controls and evaluated in three public CVID transcriptomic datasets.</p>Results<p>In this small discovery cohort, CVID was associated with widespread, cell type-specific methylation differences in T-cells and monocytes. T-cells showed a predominance of hypomethylated cytosines and regions, whereas monocytes displayed a more mixed pattern with roughly balanced hypo- and hypermethylation at individual sites.