Acoustic Cavitation-Enhanced Remodeling of Lymph Node Microenvironment Enables Deep Infiltration for Potent Cancer Vaccination

This process enables deep parenchymal infiltration of vaccine components that would otherwise be excluded. Beyond the physical barrier disruption, RNA sequencing reveals that acoustic stimulation drives both structural and immunological reprogramming of LN into an immune-permissive niche. By synergizing mechanical stimulation with biological modulation, this strategy induces benign and persistent LN expansion, amplifies anti-tumor T-cell responses, and establishes durable immunological memory, resulting in both prophylactic and therapeutic outcomes.

This approach is versatile across both model antigens and neoantigens, offering a bio-inspired, two-stage LN-targeted delivery strategy that serves as a framework for designing more effective therapeutic vaccines.