Omics · study · 2026
Time-series RNA-Seq for hMSC-TERT4 to osteoblast differentiation
Listed in NCBI GEO
Purpose: To use Next-Gen Sequencing (NGS) to generate comprehensive temporal maps of the transcriptome in hMSC-TERT4 cells induced to differentiate into osteoblasts
Description
Abstract
Alternative splicing (AS) is fundamental to our understanding of the transcriptional regulation of cell differentiation, yet the dynamic landscape of AS and its regulation during osteoblast differentiation from bone marrow stromal stem cells remains largely uncharacterised. By combining deep RNA sequencing with proteomic profiling of differentiating osteoblasts, we explore the multifaceted consequences of alternative splicing in the transcriptome, proteome and phosphoproteome.
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The consequences of AS synergise with those of gene expression, forming an integrated transcriptional program which proceeds in a multiphasic fashion. Using single-cell RNA sequencing, we show that intermediate pre-osteoblastic states and emerging cellular subtypes contribute to heterogeneity in splicing and expression patterns. These distinct subpopulations are demarcated by defined sets of genes and their isoforms, which may inform the therapeutic use of their stromal progenitors.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE181nnn/GSE181218 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE181218 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA748698 ncbi.nlm.nih.gov/bioproject/PRJNA748698 ↗
project · from NCBI GEO
- PubMed 42286000 pubmed.ncbi.nlm.nih.gov/42286000 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Bioinformatics and computational biology 70% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE181218 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3102 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |