Integrated Analysis of lncRNA-miRNA-mRNA Interaction Network in Myocardial Ischemia-Reperfusion Injury During Cardiopulmonary Bypass Surgery in Mouse [RNA-seq]

The Long non-coding RNA (LncRNA) miRNA competing endogenous RNA (ceRNA) regulatory network play a pivotal role in the intricate orchestration of cardiovascular diseases, governing their onset and progression.In order to delve deeper into the ceRNA mechanism underlying myocardial ischemia-reperfusion injury (MIRI), we conducted a randomized division of six 8-week-old male C57BL/6J mice into two groups: the Sham group and the ischemia/reperfusion (I/R) group.

Subsequently, we employed the Langendorff isolated heart perfusion system to carry out the experiment. Following the completion of the experiment, cardiac samples were meticulously collected and evenly partitioned into two segments: one for lncRNA library sequencing and the other for small RNA library sequencing. By leveraging the sequencing data, we meticulously scrutinized the disparities in lncRNA, miRNA, and mRNA expression profiles between the two sample sets, thereby unearthing the lncRNA-miRNA-mRNA ceRNA regulatory network that assumes a pivotal regulatory role in MIRI.