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Table · dataset · 2018

Data for Millennia of genomic stability within the invasive Para C Lineage of Salmonella enterica. Archive package of additional figures, tables and data

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Salmonella enterica serovar Paratyphi C is the causative agent of enteric (paratyphoid) fever.

Description

While today a potentially lethal infection of humans that occurs in Africa and Asia, early 20th century observations in Eastern Europe suggest it may once have had a wider-ranging impact on human societies. We recovered a draft Paratyphi C genome from the 800-year-old skeleton of a young woman in Trondheim, Norway, who likely died of enteric fever.

Analysis of this genome against a new, significantly expanded database of related modern genomes demonstrated that Paratyphi C is descended from the ancestors of swine pathogens, serovars Choleraesuis and Typhisuis, together forming the Para C Lineage. Our results indicate that Paratyphi C has been a pathogen of humans for at least 1,000 years, and may have evolved after zoonotic transfer from swine during the Neolithic period.<br><br>Package of additional information contains: Ancestral reconstruction 1 - Maximum likelihood phylogeny and geographical origins derived from 61,451 non-repetitive core SNPs within the Para C Lineage plus Birkenhead Ancestral reconstruction 2 - Geographic inference of the sources of clades within the Para C Lineage Concoct 1 - Single core gene (SCG) frequencies in the 76 CONCOCT clusters generated after binning contigs Concoct 2 - Statistics after clustering all metagenomes with MEGAHIT Concoct 3 - Taxonomic assignments for 11 metagenome assembled genomes (MAGs) Concoct 4 - Single-stranded deamination rates for Ragna, human DNA and 11 MAGs Concoct 5 - Genomic coverage of 11 MAGs by source and sequencing library Concoct 6 - Bacterial taxa found by Kraken in SK152 metagenomes at ≥ 0.02% frequency.

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Concoct 7 - Eleven MAGs Contigs. Concoct 8 - Alignments and phylogenies of SCGs across the Tree of Life. Date estimation 1 - Date randomization tests for the temporal signal in multiple replicate data sets that were analysed by BEAST Date estimation 2 - Linear regression plots (TempEst [37]) of root-to-tip genetic distances to the MRCA of two sets of modern genomes plus Ragna (red), Tepos-14 and Tepos-35 (green) [19] versus their dates of sampling.

Date estimation 3 - Comparison of MRCA dating estimates by BEAST using ancient plus modern, and only modern strains Date estimation 4 - Raw Maximum Clade Credibility trees as well as a summary of the mutation rates from BEAST inferences. ParaC Pan-genome 1 - Gain and loss of genomic islands shown on a maximum-likelihood radial phylogeny derived from 61,451 non-repetitive core SNPs within the Para C Lineage plus Birkenhead ParaC Pan-genome 2 - Summary statistics for pan-genomic contents of Ragna plus 219 modern genomes of the Para C Lineage ParaC Pan-genome 3 - Gene gain/loss events and numbers of pseudogenes by sub-lineage ParaC Pan-genome 4 - The pan-genome of the Para C Lineage based on 6,665 single copy genes from 220 genomes.

ParaC Pan-genome 5 - Sequences, original gene names and annotations of the reference sequences for 6,665 pan genes in the Para C Lineage. ParaC Pan-genome 6 - Pseudogenes associated with sub-lineages and clades of the Para C Lineage. ParaC Pan-genome 7 - Genomic islands and plasmids.

ParaC Pan-genome 8 - Data and script for extracting rate of gene gain/loss and pseudogenes from the branches of an ML tree. See readme.txt for instructions. ParaC genome SNPs 1 - Numbers of mutational and recombinational SNPs per clade within the Para C Lineage plus Birkenhead ParaC genome SNPs 2 - SNPs in the core genome of the Para C Lineage plus Birkenhead.

ParaC genome SNPs 3 - Maximum likelihood phylogeny and inferred geographical origins based on 61,451 non-repetitive core SNPs from the Para C Lineage plus Birkenhead. ParaC genome SNPs 4 - Venn diagram of substitutions scored as recombinational by RecHMM, ClonalFrameML and Gubbins. Salmonella supertree 1 - Metadata for 2,964 representative genomes in Salmonella enterica subsp.

I. Salmonella supertree 2 - A species tree (ASTRID) based on 3,002 core gene trees from 2,964 representative genomes from S. enterica subsp. I. Salmonella supertree 3 - A RAxML maximum likelihood phylogeny based on a 2.8 Mbp concatenate of 3,002 core genes from 2,964 representative genomes from S. enterica subsp. I Workflow - Workflow to reconstruct the Ragna genome

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Sequencing 75%
Provenance · 1 source records, 17 field assertions
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