Expression data from OT-I CD8 T cells that are either naïve or isolated on day 5 following immunization with DC-OVA or with DC-OVA + LCMV

To separate the effects of TCR/co-stimulation from the effects of inflammatory cytokines, we took an immunization approach. We transferred 1 x 10^4 OT-I CD8 T cells to naive C57BL/6 mice on day -1.

On day 0 mice were immunized with either 5 x 10^5 OVA peptide loaded, LPS-matured dendritic cells (DC-OVA) injected ip or 5 x 10^5 DC-OVA and 2 x 10^5 pfu of LCMV injected ip. On day 5 following the indicated treatment, OT-I CD8 T cells were enriched via AutoMACS and sorted via flow cytometry prior to RNA extraction from 1 x 10^6 cells using an RNeasy kit (Qiagen). Naive OT-I CD8 T cells were enriched and sorted for RNA extraction to serve as a control.