Omics · study · 2026
Charting Novel Cellular Subpopulations in the Renal Cortex of Diabetic Nephropathy in a Mouse Model Through Single-Cell RNA Sequencing
Listed in NCBI GEO
Type 1 Diabetes Mellitus (T1DM) is a prevalent autoimmune disorder character ized by the destruction of insulin-producing cells, often leading to diabetic kidney disease (DKD).
Description
Despite extensive research, the cellular mechanisms underpinning DKD remain poorly understood. This study aims to elucidate the cellular het erogeneity and specific cell populations involved in the renal cortex of diabetic mice using single-cell RNA sequencing.
Kidney cortex cells from streptozotocin (STZ)-induced diabetic mice and healthy controls were isolated and subjected to single-cell RNA sequencing. Statistical analyses were performed to identify distinct cellular subsets and evaluate changes in cell population dynamics. Immunohistochemistry and renal function evalua tions via Masson and Hematoxylin and Eosin staining were employed to validate the sequencing data.
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We first identified 11 distinct cellular subsets within the renal cortex. Diabetic mice exhibited a significant increase in distal convoluted tubule cells compared to controls. Additionally, a marked decrease in immune-related cells, including T and Blymphocytes, neutrophils, and macrophages, was observed.
Cell communication analysis highlighted the role of the VISFATIN signaling pathway, particularly the Nampt-Insr gene interaction, in the diabetic renal cortex. Our findings reveal a unique cellular and molecular landscape in the renal cortex of diabetic mice, emphasizing altered cell populations and signaling path ways critical in DKD progression. This study enhances our understanding of T1DM-induced renal alterations and suggests potential targets for therapeutic intervention to mitigate kidney complications in diabetes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE301nnn/GSE301966 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE301966 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1287798 ncbi.nlm.nih.gov/bioproject/PRJNA1287798 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE301966 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |