Omics · study · 2026
Single-Cell Cas12a Platform for Screening and CRISPRi
Listed in NCBI GEO
To overcome the challenges of pre-crRNA sequence recovery during single-cell RNA sequencing, we optimized guide RNA expression vectors and established a degron-based, enhanced Cas12a system for scalable genetic perturbation.
Description
Using this new platform, we performed Perturb-seq screens to accurately capture pre-crRNAs and profile gene editing-induced effects on the transcriptome across multiple cell types and target genes.
This dataset provides the single-cell transcriptomic profiles demonstrating the efficacy of our improved Cas12a and HyperLbCas12a systems for highly multiplexed gene knock-out and transient suppression.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE283nnn/GSE283574 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE283574 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1194560 ncbi.nlm.nih.gov/bioproject/PRJNA1194560 ↗
project · from NCBI GEO
- PubMed 42230636 pubmed.ncbi.nlm.nih.gov/42230636 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE283574 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |