Omics · study · 2026
Bulk RNA-seq profiling of human PBMCs following repeated versus single CD3/CD28 stimulation
Listed in NCBI GEO
This study investigates transcriptional differences in human peripheral blood mononuclear cells (PBMCs) subjected to repeated or single CD3/CD28 antibody stimulation.
Description
In the repeated-stimulation condition, PBMCs underwent five rounds of CD3/CD28 stimulation over 10 days, and the T-cell population was characterized by PD-1 and TIM-3 double positivity, consistent with an exhaustion-associated phenotype. In the single-stimulation condition, PBMCs received one round of CD3/CD28 stimulation for 3 days, and the activated T-cell population was characterized by CD69, CD25 and PD-1 positivity with TIM-3 negativity.
Bulk RNA sequencing was used to compare gene-expression profiles between these conditions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346542 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346542 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1525728 ncbi.nlm.nih.gov/bioproject/PRJNA1525728 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346542 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |