Single-cell RNA sequencing of knee joint synovium from mice with collagen- and antibody-induced arthritis (C&AIA)

Commonly used mouse models each carry practical constraints: collagen-induced arthritis (CIA) is largely restricted to the DBA/1 strain, whereas collagen antibody-induced arthritis (CAIA) is limited by antibody cost. To combine strain flexibility with a robust and reproducible effector phase, we established collagen- and antibody-induced arthritis (C&AIA) on the C57BL/6 background.

To characterize the synovial cellular landscape of this model, we performed droplet-based single-cell RNA sequencing on synovial tissue dissected from inflamed knee joints of arthritic C&AIA mice. This dataset resolves the immune and stromal compartments of the C&AIA synovium and enables cross-species comparison with human RA synovial single-cell data.