RNA-sequencing data of the spike in low light stress (during young microspore) and early grain filling stage (20 days after low light treatment) under low light stress (S) and control (CK) conditions

To investigate the molecular mechanisms underlying spike fertility and the opening of the glume, RNA-seq analysis was performed using the spike tissues of the ‘HN825’ and ‘KN9204’ cultivars collected on the fifth day of low light stress and at early grain filling stage (20 days after removing low light). Two biological replicate samples were collected for RNA-seq. Total RNA was extracted using Plant RNA Purification Reagent (Invitrogen).

The cDNA libraries were constructed using a TruseqTM RNA sample prep Kit (Illumina) according to the manufacturer’s instructions. RNA sequencing was performed by Shanghai Majorbio Bio[1]pharm Technology Company using an Illumina NovaSeq 6000