Omics · study · 2026
Fibrosis process activation in patients with acute cardiac rejection: A novel noninvasive diagnostic approach
Listed in NCBI GEO
Cardiac allograft fibrosis is an important limiting factor for long-term graft survival.
Description
However, the fibrotic process operating in patients with acute cellular rejection (ACR) remains unclear. We aimed to identify serum mRNAs related to cardiac fibrosis whose relative abundance become altered in patients with ACR and to evaluate their diagnostic accuracy in detecting rejection episodes.
A total of 40 serum samples from recipients of transplants undergoing routine endomyocardial biopsies were included in an RNA sequencing analysis that included 28 diagnosed with ACR (grade 1R, n=16; and grade ≥2R, n=12) and 12 without cardiac rejection. We detected several altered mRNAs associated with fibrosis in patients with ACR. Specifically, the activators of fibroblasts and myofibroblasts (TNS1, FAP and ACTA2; p<0.05), TGF-β signaling (TGFBR1 and JAK1; p<0.05) and WNT signaling (WNT7A and WLS; p<0.01) pathways showed significant differences in their relative abundance when we compared grade ≥2R ACR and/or grade 1R ACR groups with the nonrejection group.
Read the rest (2 more)
Further, TNS1 and WLS presented an area under the curve value >0.90 for identifying patients with moderate and severe grades of cardiac rejection. In conclusion, we found alterations in the relative abundance of circulating activators of fibroblasts and myofibroblasts, such as FAP or ACTA2, as well as in major profibrotic pathways, including TGF-β and WNT signaling, especially in clinically relevant cardiac rejection. These findings may contribute to improving the surveillance of patients with cardiac transplant and provide new therapeutic strategies for targeting fibrosis.
Future studies in larger cohorts are needed to better understand the potential impact of confounding variables.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315025 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315025 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1394896 ncbi.nlm.nih.gov/bioproject/PRJNA1394896 ↗
project · from NCBI GEO
- PubMed 42351799 pubmed.ncbi.nlm.nih.gov/42351799 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Homo sapiens · Non-coding RNA profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Heart 65% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE315025 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:non-coding-rna-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |