The therapeutic potential of gut microbiota-derived metabolite 4-phenylbutyric acid in Escherichia coli (E.coli)-induced colitis

The aim of this project is to use a multi omics approach combining 16S rRNA sequencing and metabolomics to analyze and identify key gut microbiota and metabolites that affect the process of E. coli infection, and further elucidate the mechanism by which gut microbiota and their metabolites regulate intestinal inflammation caused by E. coli infection through the TLR4/MyD88/NF - κ B pathway. This will provide important guidance for the development of drugs for the treatment of E. coli disease.

The samples for this experiment are calf feces, including the diarrhea group and the healthy group (without obvious diarrhea), with 6 samples in each group. The samples was sourced from a large-scale dairy farm in Yunnan, China, and the sampling subjects were newborn cows aged 3-6 months. The collection method is as follows: after wearing personal protective equipment, use fully disinfected and cleaned tools (gloves, shovels, spoons, straws) to collect samples from fresh feces center, place them in a clean and sealed container, record the sampling date, time, calf number and other information, and record relevant data.

After sampling, divide and store in the refrigerator for refrigeration.