Data · dataset · 2026
Sequencing data — [RNA‑seq and Analysis Project]
Listed in ScienceDB
This dataset is derived from the reference‑guided eukaryotic transcriptome‑sequencing project of soybean (Glycine max) leaf samples provided by the Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences.
Description
The dataset contains complete full‑pipeline data products ranging from raw image signals to final biological interpretation. Specifically, it includes high‑throughput raw sequencing data, filtered high‑quality clean data, genome‑mapping results, gene expression quantification matrices, lists of differentially expressed genes, as well as results of in‑depth functional annotation and enrichment analysis for 12 soybean leaf samples.
Specific parameters and data formats: The sequencing experiment was performed on the Illumina NovaSeq 6000 high‑throughput sequencing platform. Library construction strictly followed the standard operating procedure of the Illumina Truseq™ mRNA Sample Prep Kit. Paired‑End (PE) sequencing strategy was adopted with a read length of 2 × 150 bp.
Read the rest (2 more)
The raw off‑machine sequencing data (Raw Data) are stored in FASTQ format. The total number of raw reads (sum of both ends) is approximately 252 million, and the total raw bases reach around 76 Gb. After rigorous quality‑control filtering, about 75 Gb of clean data and 251 million clean reads were obtained.
The clean reads of each sample stably ranged from 20 million to 25 million (see Table 1 for details). The sequencing error rate was kept at a very low level. The percentage of Q30 bases (base‑calling accuracy ≥ 99.9 %) of all samples was higher than 92 %, and Q30 values of some samples reached as high as 97.72 %, which fully demonstrates the high output and high accuracy of this batch of sequencing data.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.iga.001du ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Image 75% · Plant biology 75% · Sequencing 75% · Tabular 65%
Provenance · 1 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.IGA.001du | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3108 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:tabular | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |