Data · dataset · 2026
Knockout of hsd3b1 leads to disrupted steroid hormone homeostasis and compromised gonadal development in zebrafish
Listed in ScienceDB
Steroid hormones play crucial roles in gonadal development, and defects in steroid synthesis can lead to various metabolic diseases and reproductive disorders.
Description
Among these, 3β-hydroxysteroid dehydrogenase (3β-HSD), encoded by the HSD3B gene, catalyzes the conversion of inactive 5-double bond 3-beta hydroxysteroid precursors to active 4-double bond ketosteroids, representing a critical step in steroidogenesis.
In this study, we successfully generated the hsd3b1 mutant zebrafish using CRISPR/Cas9, and the mutant zebrafish display abnormal gonadal development, interrenal hyperplasia, hyperpigmentation and reduced reproductive capacity. RNA sequencing and targeted metabolomic analysis of steroid hormones revealed that steroid hormone homeostasis was disrupted in hsd3b1 mutants, characterized by reduced metabolic differences between males and females.
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These findings indicate that hsd3b1 is essential for gonadal development and steroid hormone regulation in zebrafish. The hsd3b1 mutant provides a robust model for investigating the mechanisms linking steroidogenesis to reproductive development in vertebrates.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.35916 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.35916 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |