Omics · study · 2026
Distinct media requirements for dopaminergic neuron reprogramming from human glia and fibroblasts
Listed in NCBI GEO
Bulk RNA sequencing was performed on human glial progenitor cells (hGPCs) derived from the hESC line RC17 and reprogrammed toward a dopaminergic neuron fate using Ascl1, Lmx1a, Nurr1 and shREST (ALNRi).
Description
Cells were cultured under two media conditions; standard NDiff medium or a B27/Neurobasal-based medium without small molecules (new media) and harvested at day 10 and day 21 post-transduction (three technical replicates per condition).
The dataset was generated to identify transcriptional differences underlying the improved dopaminergic reprogramming efficiency observed with the new media. Gene-level counts were quantified using Salmon (alignment-based mode, GRCh38/hg38) and merged across samples using tximport.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333803 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333803 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1472565 ncbi.nlm.nih.gov/bioproject/PRJNA1472565 ↗
project · from NCBI GEO
- PubMed 42608437 pubmed.ncbi.nlm.nih.gov/42608437 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 69% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE333803 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |