Excel · study · 2026
Transcriptomic profiling of primary human prostate cancer-associated fibroblasts (CAFs) in response to ADT and Enzalutamide
Listed in NCBI GEO
Cancer-associated fibroblasts (CAFs) are critical components of the prostate tumor microenvironment and modulate therapeutic responses.
Description
In this study, we aimed to investigate the transcriptomic alterations in primary human prostate cancer CAFs subjected to different anti-androgen treatments. Primary CAFs were isolated from human prostate cancer tissues and cultured in vitro.
The cells were divided into three treatment groups: Vehicle (control), Androgen Deprivation Therapy (ADT), and a combination of ADT plus Enzalutamide (ADT_Enza). High-throughput RNA sequencing (RNA-seq) was performed on 9 samples (3 biological replicates per group) to identify differentially expressed genes and underlying molecular mechanisms. This dataset provides a comprehensive stromal transcriptomic landscape, offering insights into how CAFs react to standard-of-care prostate cancer therapies.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328022 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328022 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1453527 ncbi.nlm.nih.gov/bioproject/PRJNA1453527 ↗
project · from NCBI GEO
- PubMed 42244988 pubmed.ncbi.nlm.nih.gov/42244988 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 68% · Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE328022 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |