ChIP-seq analysis of IRF1 binding in VSV-infected bone marrow-derived macrophages (BMDMs)

BMDMs were differentiated from mouse bone marrow cells via M-CSF stimulation and then infected with VSV (MOI = 1) for 4 hours or mock-treated. Chromatin immunoprecipitation (ChIP) was performed using an anti-IRF1 antibody, followed by next-generation sequencing (Illumina).

Data analysis included alignment to the mm10 genome, peak calling, and coverage normalization to identify IRF1 binding sites under viral infection. These results provide insights into IRF1's regulatory role in antiviral immune responses.