Hopx is a dual-gatekeeper of astrocyte fate enabling in vivo reprogramming and neural repair

The analysis revealed Hopx knockdown-associated induction of neurogenic and neuronal transcriptional programs in AAV-transduced astrocyte-lineage cells, including increased expression of progenitor- and neuron-associated markers and gene modules related to generation of neurons and neuron fate commitment. These data support the conclusion that Hopx repression promotes a progressive transcriptional transition from a glial state toward a neuron-like state and provide a transcriptomic resource for studying endogenous regulation of astrocyte plasticity during in vivo neural reprogramming.

In parallel, we performed HOPX ChIP-seq to define the genome-wide binding landscape of HOPX and identify HOPX peak-associated candidate genes. Together, the scRNA-seq and ChIP-seq datasets provide complementary resources for investigating the transcriptional and genomic regulatory mechanisms underlying astrocyte-to-neuron conversion in vivo.