Multi-tissue transcriptome sequencing of Quasipaa spinosa from Fujian Province, China for genome annotation

The transcriptomic data were generated to support genome annotation of a newly assembled chromosome-scale reference genome. Ten adult tissues, including brain, heart, liver, kidney, lung, intestine, skin, spleen, ovary, and testis, were sampled, and total RNA was extracted separately from each tissue.

Equal amounts of high-quality RNA from all tissues were pooled to construct a comprehensive RNA sequencing library. The resulting transcriptome dataset was generated using the DNBSEQ sequencing platform and provides expressed sequence evidence for protein-coding gene prediction and functional annotation. These data complement long-read genome sequencing resources, including PacBio HiFi, Oxford Nanopore ultra-long reads, and Hi-C sequencing, and contribute to the development of genomic resources for Q. spinosa population genetics, comparative genomics, conservation studies, and selective breeding.