Transcriptomic analysis of hippocampus and prefrontal cortex in a mouse model of chronic radiation-induced gastrointestinal injury

To investigate molecular changes in the brain during chronic radiation-induced intestinal injury, RNA sequencing was performed using hippocampal and prefrontal cortex tissues collected from irradiated mice and non-irradiated controls.

Transcriptomic profiling revealed region-specific alterations, with the hippocampus showing more pronounced changes in pathways related to vascular remodeling, extracellular matrix organization, inflammatory response, and blood–brain barrier dysfunction. These datasets provide a resource for understanding gut–brain axis-associated molecular alterations in chronic radiation-induced injury.