Omics · study · 2026
Single-cell RNA sequencing of mouse peripheral blood neutrophils before and one day after corneal injury
Listed in NCBI GEO
Neutrophils are rapidly mobilized into circulation following sterile tissue injury, but the transcriptional heterogeneity of the peripheral blood neutrophil pool during this response is incompletely defined.
Description
To characterize how corneal injury reshapes the circulating neutrophil compartment, we performed droplet-based single-cell RNA sequencing on FACS-sorted peripheral blood neutrophils from mice at steady state (Pre-injury, normal) and one day after corneal injury (Day 1 post-corneal injury).
Comparative analysis resolved distinct neutrophil states and revealed injury-associated shifts in subset composition. These data define the circulating neutrophil response to remote sterile corneal injury at single-cell resolution.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338556 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338556 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1495146 ncbi.nlm.nih.gov/bioproject/PRJNA1495146 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE338556 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |