Discovery and application of novel antimicrobials against the protozoan Histomonas meleagridis: the causative agent of Histomonosis in poultry

It was found that there was found that the Primers tested either did not amplify an amplicon at the correct size or produced amplicons for samples which did not contain H. meleagridis. To try and help with future detection there was an attempted to sequence the genome. There was issues with this and contamination plagued the isolation and sequencing of the genome resulting in only 5% of the genome being sequenced. <br><br>In total 82 AMPs were tested successfully this was though developing a method for high throughput testing of compounds for microbes which cannot be cultivated in isolation using microscopy. 16 AMPs had a lower susceptible concentration compared to the last control compound and will be investigated further.

Rumen derived AMPs were tested to directly affect H. meleagridis with activity at the same concentration in the initial testing. In conclusion the study has identified and potentially found a way to treat H. meleagridis, which now requires in vivo confirmation.