Transcriptional profiling of Clarke's column neurons after Pv>DTA-mediated sensory deprivation.

Alexa555-CTB was injected into the cerebellum at postnatal day 4 (P4). At P6, spinal cord tissue was dissociated using pronase in ACSF, followed by mechanical trituration with Pasteur pipettes, and labeled CC neurons were manually collected using a capillary glass pipette.

Sequencing libraries were prepared from pools of 10-20 cells following the SMART-seq2 protocol. Sequencing was performed on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads. Raw sequencing reads were aligned to the Mus musculus reference genome (GRCm39) using STAR (version 2.7.11a).