16S rRNA and Sequencing data of fecal samples

Fecal samples of 31 individuals with hepatitis B virus (HBV)-infected liver cirrhosis before any therapeutic intervention (liver cirrhosis was determined by Hepatitis B, biopsy or two imaging methods (ultrasound, CT, and MRI) and 15 uninvolved healthy volunteers (without physical and psychological diseases from the people of a regular healthy physical examination every year by detecting the blood, urine and fecal routine, liver function, biochemical analysis, hepatitis markers, chest X-ray, abdominal ultrasound and so on) were acquired.

Fecal sample collection was performed by using a TinyGene fecal collection box according to the manufacturer’s instructions (TinyGene, Shanghai), and the boxes were placed on ice as soon as possible and stored at -80℃ before further analysis. The 46 samples were subjected to library preparation (including genomic DNA extraction, PCR amplification, recovery using the AxyPrep DNA Gel Extraction Kit, and real-time fluorescence quantification on the FTC-3000TM real-time PCR instrument), followed by Illumina high-throughput sequencing and bioinformatics analysis.