Effect of eIF5A K49 acetylation status on the transcriptome of Toxoplasma gondii

Using the ME49 strain as our genetic background, we performed whole-transcriptome mRNA sequencing on three distinct variants: the wild-type, an acetylation-mimic mutant (eIF5A-K49Q), and a deacetylation-mimic mutant (eIF5A-K49R).

The parasites were cultured under both standard conditions (non-stressed tachyzoites) and alkaline stress conditions (pH 8.2 medium for 4 days) to induce *in vitro* bradyzoite differentiation. With three biological replicates for each group, a total of 18 samples were analyzed. This dataset provides a robust transcriptomic foundation for gaining deeper insights into how the acetylation of eIF5A-K49 reshapes gene expression programs during the developmental stage conversion of Toxoplasma gondii.