Transcriptomic profiling of multiple tissues from aged mice with endothelial-specific Slc2a3 (GLUT3) overexpression

Glucose transporter 3 (GLUT3), encoded by Slc2a3, is a high-affinity glucose transporter that may influence endothelial metabolism and systemic tissue homeostasis. To investigate whether endothelial-specific GLUT3 overexpression modulates age-associated transcriptional changes across multiple organs, we performed RNA sequencing of tissues collected from 24-month-old wild-type (WT) mice and mice with endothelial-specific Slc2a3 overexpression (R26-LSL-Slc2a3;Cdh5-2A-CreERT2, KI).

Ten tissues, including heart, liver, spleen, lung, kidney, small intestine, brown adipose tissue (BAT), hindlimb skeletal muscle, skin, and brain, were collected from three biological replicates per group. This dataset provides a comprehensive multi-tissue transcriptomic resource for evaluating the effects of endothelial GLUT3 overexpression on age-associated molecular alterations and inter-organ responses in aged mice.